acta satech Journal of the Life & Physical Sciences Babcock

Chemomorphological study and antimicrobial activity of Daedalea quercina
*1&2 L. N. Ofodile, 3Uma N. U., 4Attah L. E. 2Simmonds M. S. J, 5 Popoola O. E.
1Department of Biological Sciences, Yaba College of Technology, Lagos, Nigeria 2Royal Botanic Gardens, Kew, Richmond TW9 3AB, UK 3Department of Botany and Microbiology, University of Lagos, Akoka, Lagos, Nigeria 4Department of Applied chemistry, Jimma University, Ambo College, Ethiopia. 5Department of Chemical Sciences, Yaba College of Technology, Lagos, Nigeria
2010

ABSTRACT
Chemical and morphological studies were carried out on Daedalea quercina Fr. from Nigeria to determine its characteristics. Fifty specimens of the basidiocarps of Daedalea quercina were observed and collected from the dead log of Mangifera indica in August 2003, Obosi, Nigeria. The specimens were characterized using microscopic and macroscopic parameters and chemical spot test. The antimicrobial activity of the crude n-hexane: diethyl ether, chloroform: acetone and methanol extracts of the polypore were tested using thin layer chromatography agar overlay method. Morphologically, D. quercina was yellowish in colour, broadly sessile, and laterally fused in 2-3 layers. Basidiocarp was leathery with a pleasant smell. The pileus was concentrically zoned, has lamellate pore surface and the hyphal system was trimitic with ellipsoid, hyaline spores. Chemically, the colour of the tissues changed to brown with Ferric chloride, non-reactive with Melzer’s reagent, and produced characteristic colours with various reagents used in the test. None of the solvent extracts inhibited the growth of C. herbarium. The three solvent extracts were active against B. subtilis but the growth of P. syringea was inhibited by the hexane: ether and chloroform: acetone extracts of the fungus. Phenolics and terpenes were detected in the three solvent extracts.
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