acta satech Journal of the Life & Physical Sciences Babcock

Effect of Anthocyanin and Ascorbic Acid in Graded Levels of Roselle (Hibiscus sabdariffa linn.) Calyx Extract on Blood Profile of Broiler Chickens
Awodola-Peters Omolade Oluwakemi1 and LONGE Oyebiodun Grace2
1Department of Animal Production, Federal College of Animal Health and Production Technology, Moor Plantation Ibadan, Oyo State. 2Department of Animal Science, Faculty of Agriculture, University of Ibadan, Oyo State, Nigeria.
June 2015

ABSTRACT
A total of 200 day old Abor acre broiler chicks were randomly assigned to five treatments and replicated four times in a completely randomized design to evaluate the effect of anthocyanin and ascorbic acid in graded levels of the red variety of roselle calyx extract on haematological indices and serum biochemistry of broiler chickens. Treatment 1 served as control, while treatment 2, 3, 4 and 5 represented the various graded levels of the extract. The experiment lasted 42 days in which feed was supplied ad libitum. All data obtained were subjected to ANOVA and means separated using Duncanís New Multiple Range Test. Haematological indices namely PCV, haemoglobin and RBC showed no significant difference (P>0.05) in the values obtained among treatment means. However, there was statistical difference (P<0.05) in the values obtained for WBC and neutrophils, other factions of WBC showed no significant difference. Total protein, albumin and AST obtained showed no significant difference (P>0.05) except for globulin and ALT. Anthocyanin and ascorbic acid present in the aqueous extract boosted immunity and fought infections when administered to broiler chickens. A graded level of 4g of the calyx boiled in one litre of water for 30minutes is however recommended for optimum result.
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