acta satech Journal of the Life & Physical Sciences Babcock

Isolation of Bacterial Pathogens from Selected Door Handles in a Southwestern University in Nigeria
Mensah-Agyei, G. O.1; Okeke G. A.1; Oyewole I. O1; and Osopale B.A.1
1 Department of Biosciences and Biotechnology, School of Basic and Applied Science Babcock University, Ogun State, Nigeria.
September 2016

ABSTRACT
Background: One major cause of concern in public health is the increase in epidemic outbreaks and its spread through several communities. In time past, door knobs among other formites has posed as one significant route of transfer of the causal organism epidemic outbreaks. Methodology: Door knobs of toilets, offices and rooms in Babcock University were evaluated to determine the level of bacterial contamination and to identify the bacteria contaminants. Eighty (80) swab samples were obtained from various doorknobs of offices, halls of residence and toilets. The swab samples were inoculated, pure cultures were obtained, Gram stained and biochemical tests were carried out on the isolates for identification. Result: Seven bacterial contaminants were isolated. These organisms includes P. aeruginosa which had the highest prevalence 32.1%, followed by K. pneumoniae (20.5%), Bacillus species (11.6%), Proteus species (11.6%), Neisseria species (9.8%), while E. coli (7.1%) and S. aureus (7.1%). This shows that door handles are potential carriers of pathogenic bacteria which are capable of causing epidemics. Conclusion: Therefore, a combined effort of governmental, private organizations and individuals to educate the populace on personal and environmental hygiene is necessary to prevent spread of disease through door handles.
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