acta satech Journal of the Life & Physical Sciences Babcock

Toxicological evaluation of leaf essential oil of Citrus sinensis on alloxan-induced diabetic rats
*Soji-Omoniwa, O.1; Omoniwa, B.P.2 and Usman, L.A3
1 Department of Biochemistry, University of Ilorin, Nigeria. 2 Ethnopharmacology/Toxicology Laboratory, Department of Science Laboratory Technology, University of Jos, Nigeria. 3 Department of Chemistry, University of Ilorin, Nigeria
3rd of April 2017

ABSTRACT
This study investigated the toxicity effect of leaf essential oil of Citrus sinensis (Rutaceae) on alloxan –induced diabetic rats, having confirmed its antidiabetic potential in previous study. Forty (40) male albino rats were randomly selected into 4 groups ( A, B, C and D) of 10 rats each, representing (respectively) the Normal Control, Diabetic Control, Diabetic treated with 14.2 mg/kg b. wt. Metformin (reference drug), and Diabetic treated with 110 mg/kg b.wt. leaf essential oil of Citrus sinensis. Experimental animals apart from the normal, diabetic, and referenced drug treated groups were treated with the 110 mg/kg b.wt of the oil for 15 days. Effect on hematological parameters, liver and kidney function tests were evaluated. Results showed that there was a significant increase (p<0.05) in the concentration of red blood cell, packed cell volume and no significant difference (p<0.05) in White blood cell, lymphocyte and neutrophil when compared to the normal control. Citrus sinensis essential oil-treated diabetic rats also showed a significant increase (p<0.05) in total protein concentration, significant decrease (p<0.05) in albumin, total bilirubin and direct bilirubin concentrations compared to the normal control. Concentrations of serum creatinine and urea in the essential oil-treated diabetic rats significantly increased and decreased respectively when compared with the normal control group. A significant increase (p<0.05) was observed in the activity of heart alanine transaminase. Results of this study suggest that the leaf essential oil of Citrus sinensis at a dose of 110 mg/kg b.wt administered for 15 days might be toxic to the tissues study.
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