acta satech Journal of the Life & Physical Sciences Babcock

Phytochemical Screening and Antimicrobial Testing of Vernonia Amygdalina Del. Leaves on Salmonella typhi
R. O. Omenka, I. O. Oyewole & A. O. Kayode
Department of Basic and Applied Sciences, Babcock University, Ilishan-Remo, Nigeria
Received October 2nd, 2003, revised version received February 2nd, 2004, accepted February 18th, 2004

ABSTRACT
The preliminary phytochemical screening of Vernonia amygdalina Del (leaves) showed the presence of the following bioactive agents: saponins; alkaloids; phlobatannins; tannins and cardiac glycosides. Phlobatannins, flavonoids and anthraquinones were absent in ethanolic extract while tannins, flavonoids and anthraquinones were absent in crude extract. Antimicrobial sensitivity testing was performed on Salmonella typhi using different extracts of Vernonia amygdalina leaves and compared with a drug, chloramphenicol. The diameters of the Zones of inhibition on the disc diffusion sensitivity test were 12.0� 0.4 for the ethanolic extract of the dry, powdered leaves, 8.2� o.6 for the fresh leaf aqueous extract, 9.7� 0.5 for the dry leaf aquenlus extract, 38.5� 0.5 for chroramphenicol (1000?g/ml) and 0 for the control. These results showed that V. amygdalina possesses a degree of antimicrobial activity against S. typhi �in vitro�
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